4 cells used in cartilage repair and tissue engineering
Transplantation and improvement of mouse embryo progenitor derived insulin producing cells for type 1 diabetes therapy
... as a coordinate partner to insulin by reducing food intake, slowing gastric empty and inhibiting digestive secretion in short term Due to the release of insulin, C-peptide and amylin, -cells ... and Ins2 in rodents) and synthesis of insulin is achieved by bio-processing from its precursors, preproinsulin and proinsulin, which ensures the production of sufficient amounts of mature insulin ... of CNC and the small Maf proteins (87-90) In contrast to the small Mafs, the large Maf proteins including MafA, c-Maf, MafB and Nrl contain a transactivator domain in their amino terminus and activate...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response to elevated glucose concentration ... LC: Distinct distribution of laminin and its integrin receptors in the pancreas J Histochem Cytochem 2002, 50(12):1625-1632 Hulinsky I, Harrington J, Cooney S, Silink M: Insulin secretion and DNA...
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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
... 6:42 line of defence against a pathogen attack and rapidly activate defence signalling pathways following initial infection TLRs are considered as playing a crucial role in the switch from innate ... situation prevailing in the presence of TLR2 ligand TLR7 and triggering resulted in little impact on IκBα, which is not surprising considering the reported low expression levels of TLR7 and in IM-MDDCs ... Actin Flagellin R837 15 30 Unmethylated DNA 15 30 15 30 I B -P I B Actin NF-κB is activated in IM-MDDCs following TLR2, and Figure triggering NF-κB is activated in IM-MDDCs following TLR2, and...
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Mypt1 mediated spatial positioning of bmp2 producing cells is essential for liver organogenesis
... venous blood and supplied by the portal vein that drains the blood from the intestinal system (including the pancreas and the spleen), rich in nutrients and poor in oxygen The remaining blood supply ... molecules or by mediating intracellular signal through interaction with integrins β1-integrin is a component of receptor for ECM proteins laminins and collagens The β1-integrin knockout embryonic ... foregut and invade as cords into the surrounding septum transversum mesenchyme (Douarin, 1975; Medlock and Haar, 1983) The hepatoblast cords intermingle with the vitelline veins, anastomosing into...
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Báo cáo y học: " Production of infectious human immunodeficiency virus type 1 does not require depletion of APOBEC3G from virus-producing cells" pdf
... protein Frequently, epitopes are masked because of the folding properties of a protein in vivo or because of pre-existing protein-protein interactions that may compete for antibody binding To ... activity and used for the infection of LuSIV indicator cells Relative virus infectivity was determined by comparing the Tat-dependent expression of luciferase in the target cells (Fig 7) Interestingly, ... initial findings and suggest that – instead of inducing APOBEC3G degradation – Vif induces conformational changes in APOBEC3G that affect the ability of antibodies to interact with the protein (manuscript...
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Investigation of the roles of two rac1 effectors phosphatidylinositol 5 kinase 1a and p21 activated kinase 1 in insulin secreting cells
... reviewed in (68) and compared in (69) Currently, there are a number of widely used insulinsecreting cell lines These includes RIN (70), HIT (71), BRIN-BD11 (72), MIN6 (73) and INS-1 cells (74) In 1992, ... involved in mediating the action of Rac1 on actin cytoskeleton remodeling In this thesis study, their potential roles in insulin secretion and cell survival were investigated in INS-1 cell line, ... differentiated insulinsecreting cell line, INS-1, from cells isolated from X-ray-induced rat insulinoma (74) INS-1 cells, containing the highest insulin content (around µg/106cells, corresponding to...
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ROLES OF HUNTINGTIN ASSOCIATED PROTEIN 1 IN INSULIN SECRETING CELLS
... HAP1 and insulin HAP1 is also expressed in INS-1 cell line, a commonly -used insulin-secreting cell line from rat insulinoma Western blotting further confirms that there are two HAP1 isoforms in INS-1 ... produce insulin by themselves, thus have to inject insulin substitute to maintain glucose homeostasis On the other hand, type patients result from relative insulin deficiency and insulin resistance, ... of insulin-secreting cell lines have been created by induced insulinomas, viral transformation, and transgenic mice [34] Among them, the INS-1 cell line from rat insulinoma is a most widelyused...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... follows: aprotinin, leupeptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industries (Osaka, ... (N-(R)(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-l3-(2¢-naphthyl)alaninyl-l-alanine 2-aminoethyl amide; TAPI-1) did not affect the processing A commercially available proteinase inhibitor mixture containing AEBSF, aprotinin and some other inhibitors inhibited ... [4,5,8] and stimulates cell growth in culture medium containing insulin or IGFs [9,10] The IGFBP-rP1 mRNA is expressed in a wide range of normal tissues including the heart, spleen, ovary, small intestine...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
... with amino acids added) containing 2% D-glucose, and the cultures diluted to an D600 of 0.5 in synthetic medium containing 0.5% D-glucose and grown further with shaking at 30 °C Upon reaching an ... to a ®lter containing soluble protein extracts obtained from wild-type and mls1D cells that were propagated in rich medium supplemented with ethanol This resulted in a protein band with a molecular ... compartment in cells grown on glucose, Mls1p was selectively retained in the cytosol of cells propagated on ethanol Interestingly, the C-termini of both Mls1p and Aat2p contain acidic amino-acid...
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... protease and protein phosphatase inhibitors, frozen within 10 s, and thawed in boiling water to minimize postincubation signalling modifications in the cells and protein modifications during immunoprecipitation ... serine ⁄ threonine phosphorylation of IRS-1 and IRS-2 inhibits their binding to the juxtamembrane region of the insulin receptor and impairs their ability to undergo insulin-induced tyrosine ... were incubated with indicated concentration of insulin for 10 Mean ± SE, n ¼ subjects In this and the following figures, the insulin-stimulated effect was obtained by setting the value with no insulin...
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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
... IGF2R-binding site, while the B domain and the ®rst A domain helix contain the major determinants for IGFBP binding In this study we show, using IGF-I, IGF-II and 14 IGF analogues, that potency in ... stimulate DNA synthesis in L7 murine ®broblasts compared with IGF-I [14] Interestingly, strong correlations between insulin or insulin mutant binding to the insulin receptor and metabolic potencies ... rhIGF1R binding by IGF-I, IGF-II and 14 analogues using BIAcore analysis IGF1R binding by all of these analogues has not previously been compared in a single binding assay Analogues included in this...
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Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells define structural determinants of receptor function doc
... eumelanins, and the reddish to yellow, sulfurcontaining phaeomelanins [17] Although both types of melanins are found in different relative proportions in all skin types, the phaeomelanin/eumelanin ... loading and transfer were ascertained by cutting the lower portion of the membrane before blocking and staining for total protein with Amido Black RESULTS AND DISCUSSION Seven HMC lines and three ... the Gs protein In many GPCRs, the third intracellular loop and the cytoplasmic C-terminal extension are the main regions involved in coupling to the G proteins [2] It is clear that in the case...
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Báo cáo khoa học: Colocalization of insulin receptor and insulin receptor substrate-1 to caveolae in primary human adipocytes Cholesterol depletion blocks insulin signalling for metabolic and mitogenic control doc
... 30] and fixed in 3% (w/v) paraformaldehyde for 30 at room temperature After blocking in bovine serum albumin and gelatine membranes were incubated with rabbit antiIRS1, caveolin, or insulin receptor ... by centrifuging through dinonylphtalate and freezing in an aluminium block kept at )20 °C Non-specific uptake was determined in the presence of 25 lM cytochalasin B by terminating the incubation ... receptors After incubating cells with a submaximal concentration of insulin the insulin-induced increase in receptor autophosphorylation was comparable in the isolated caveolae fraction and in the total...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... protease inhibitor cocktail for mammalian extracts (containing AEBSF, aprotinin, leupeptin, bestatin, pepstatin a and E-64) was obtained from Sigma (St Louis, MO) Plasmid construction and site-directed ... remain uncapped and therefore can be translated only by a cap-independent mechanism In the HCV IRES-containing constructs, initiation of transla- nt 342 ATG initiator in core ORF CMV del ATG initiator ... Frameshifting is mediated by slippage of ribosomes during translation elongation at core codons 8–11 and yields a core+1 chimeric protein containing the first 8–11 amino acids of core fused to amino...
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báo cáo hóa học:" Three-dimensional growth as multicellular spheroid activates the proangiogenic phenotype of colorectal carcinoma cells via LFA-1-dependent VEGF: implications on hepatic micrometastasis" docx
... vascular stages during hepatic colon carcinoma metastasis Materials and methods Cell line and maintenance Murine colon carcinoma cell line (CT26) was obtained from American Tissue Culture Collection ... lipoprotein, ovalbumin); negative phagocytosis (1 μm latex particles) and CD45 antigen expression; positive lectin binding-site expression (wheat germ and viscum album agglutinins); and negative ... Eto S: CD44 stimulation induces integrin-mediated adhesion of colon cancer cell lines to endothelial cells by upregulation of integrins and c-Met and activation of integrins Cancer Res 1999, 59:4427-34...
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Báo cáo hóa học: " The emerging role of insulin-like growth factor 1 receptor (IGF1r) in gastrointestinal stromal tumors (GISTs)" pdf
... Scotlandi K: Inhibition of insulin-like growth factor I receptor increases the antitumor activity of doxorubicin and vincristine against Ewing’s sarcoma cells Clin Cancer Res 2001, 7:1790-7 Martins ... [50] IGF1R signaling was blocked in many other types of sarcomas to explore its role in cell proliferation and survival in vitro, and tumor growth, invasion and metastasis in vivo in animal models ... overcome KIT and PDGFRA-dependent imatinib resistance [55] Glycemic derangements related to insulin-like growth factors such as the pro-IGF-IIE and insulin-like growth factor-binding proteins have...
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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... protein kinase PKR in the inhibition of varicella-zoster virus replication by beta interferon and gamma interferon J Gen Virol 2005, 86(Pt 1):1-6 Sainz BJ, Halford WP: Alpha/Beta interferon and ... DA: Trafficking and signaling pathways of nuclear localizing protein ligands and their receptors Bioessays 2004, 26(9):993-1004 Lucin P, Jonjic S, Messerle M, Polic B, Hengel H, Koszinowski UH: ... KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 infection Likewise,...
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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... protein kinase PKR in the inhibition of varicella-zoster virus replication by beta interferon and gamma interferon J Gen Virol 2005, 86(Pt 1):1-6 Sainz BJ, Halford WP: Alpha/Beta interferon and ... DA: Trafficking and signaling pathways of nuclear localizing protein ligands and their receptors Bioessays 2004, 26(9):993-1004 Lucin P, Jonjic S, Messerle M, Polic B, Hengel H, Koszinowski UH: ... KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 infection Likewise,...
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Báo cáo y học: "Pharmacological disruption of insulin-like growth factor 1 binding to IGF-binding proteins restores anabolic responses in human osteoarthritic chondrocytes" ppt
... 1996, 39:968-978 Morales TI: The insulin-like growth factor binding proteins in uncultured human cartilage Increases in insulin-like growth factor binding protein during osteoarthritis Arthritis ... small-molecule inhibitor of the binding of insulin-like growth factor (IGF)-1 to IGF-binding proteins (IGFBPs), with 125Ilabeled IGF-1 binding to cartilage labeled IGF-1 binding to cartilage Explants ... washes of minutes each in 10 mM Tris-buffered saline, pH 7.4, containing 0.15 M NaCl (TBS), membranes were incubated for 30 minutes in TBS containing 3% NP40 and blocked in TBS containing 0.5%...
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Báo cáo y học: "IFN-gamma regulation of ICAM-1 receptors in bronchial epithelial cells: soluble ICAM–1 release inhibits human rhinovirus infectio" pptx
... undetected in untreated HRV-14 infected cell cultures at any time point IFN-γ induced a small but significant increase in sICAM-1 release in uninfected cells and cells infected with the minor group rhinovirus, ... spectrum protease inhibitor cocktail containing serine, cysteine, metalloprotease and calpain inhibitors (Complete™ Boehringer Ingelheim, Germany) at a concentration of Complete™ mini tablet/10 ml ... infection, and may explain, albeit partly, the self-limiting nature of rhinovirus infections observed in vivo In support, sICAM1 has been shown to possess anti-viral properties both in vitro [15] and in...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25